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New red fluorescent proteins allow scientists to better 'see' what memories are made of. We describe a red-shifted fluorescence resonance energy transfer (FRET) pair optimized for dual-color fluorescence lifetime imaging (FLIM). This pair utilizes a newly developed FRET donor, monomeric cyan-excitable red fluorescent protein (mCyRFP1), which has a large Stokes shift and a monoexponential fluorescence lifetime decay.

Article DOI: 10.1038/nmeth.4046
Contributed by: Joyce Tacket

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